Congression of unlinked markers and genetic mapping in the transformation of Bacillus subtilis 168.
نویسندگان
چکیده
A thorough examination of cotransformation of two unlinked genetic markers in Bacillus subtilis 168 shows that the two recombinational events do not occur randomly. The cotransformation frequency is dependent on the distance between the two markers as well as on the order in which they replicate in the competent cell. These results indicate that uptake and/or integration of DNA molecules bearing these genetic markers is enhanced at the time these markers replicate in the competent cell.
منابع مشابه
Genetic mapping of a defective bacteriophage on the chromosome of Bacillus subtilis 168.
A genetic marker responsible for the killing activity of PBSX, a defective phage carried by Bacillus subtilis 168, has been located on the bacterial chromosome. Two mutant strains of B. subtilis 168, which produced tailless phage particles upon mitomycin C induction, were shown to carry lesions, designated xtl-1 and xtl-2, which were linked by transformation and PBS1-mediated transduction to me...
متن کاملGenetic control of flagellation in Bacillus subtilis.
Flagellation in Bacillus subtilis was shown to involve at least three loci: a gene H controlling the specificity of the flagellar antigen; a gene fla controlling the presence or absence of flagella; and a gene mot controlling the function of the flagella. The loci were shown to be nonallelic and unlinked in transformation tests. Strains W 23 and SB 108-b, a derivative of strain 168, were shown ...
متن کاملD-Tyrosine as a metabolic inhibitor of Bacillus subtilis.
The d-isomer of tyrosine is a potent inhibitor of growth in transformable strain 168 of Bacillus subtilis. A d-tyrosine-resistant mutant of the inhibited strain was isolated which excreted l-tyrosine, had a diminished growth rate, and required l-phenylalanine to attain the growth rate of the wild-type parent. Mapping by deoxyribonucleate transformation located this resistance in the gene coding...
متن کاملPhysical and genetic mapping of the SPO2 prophage on the chromosome of Bacillus subtilis 168.
It has been found by density transfer and genetic mapping experiments that prophage SPO2 is linked to the antibiotic resistance marker ery-1 in Bacillus subtilis 168.
متن کاملBacteriophage Deoxyribonucleate Infection of Competent Bacillus Subtilis.
Reilly, Bernard E. (Western Reserve University, Cleveland, Ohio), and John Spizizen. Bacteriophage deoxyribonucleate infection of competent Bacillus subtilis. J. Bacteriol. 89:782-790. 1964.-Phenol extracts of the Bacillus subtilis bacteriophages phi1, phi25, and phi29 contained infectious deoxyribonucleic acid. The infectivity was destroyed by catalytic amounts of deoxyribonuclease but not by ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Genetics
دوره 73 1 شماره
صفحات -
تاریخ انتشار 1973